Quantitative hybridization to genomic DNA fractionated by pulsed-field gel electrophoresis
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چکیده
منابع مشابه
Quantitative hybridization to genomic DNA fractionated by pulsed-field gel electrophoresis.
Hybridization to genomic DNA fractionated by CHEF electrophoresis can vary >100-fold if the DNA is acid depurinated prior to Southern blotting. The level of hybridization is high or low depending on whether the molecule being analyzed migrates at a size coincident with or different from the size of the majority of genomic DNA in the sample, respectively. Techniques that avoid acid depurination ...
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DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
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FACTORS THAT AFFECT PFG SEPARATIONS........................................................................ 294 Molecular Properties ............................................... .................................... ............. 294 Pulse Times............................................................................................................... 295 Electrical Field Shape ..............
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1998
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/26.20.4787